The E. G. Barnhill Collection contains negatives of Florida homes and birds, as well as glass plate negatives of Florida scenes. The collection also contains hand-colored postcards by Barnhill depicting Florida scenes (ca. 1910s-1930s), early Florida ephemera,  several biographical pages and scrapbooks put together by Barnhill with many resourceful historical clippings from the early half of the 20th century on pirates, Native Americans, archaeological exploits, early technology, and treasure hunting.

Collection contains a typescript by William Monroe Heine titled "A Remnant of the Seminoles," as well as a photocopy of that typescript.