The Florida Photograph Collection contains a series of photographs and negatives depicting the state from 1886 to 1950. The content of these includes people, scenery, nature, infrastructure, historic areas and landmarks, housing, commerce, tourism, and aerial views. Cities photographed include, but are not limited to, Miami, Miami Beach, Coconut Grove, St. Augustine, Key West, Coral Gables. Also of particular notice are photographs of Seminole Indians and villages, and historic photographs by Ed Romer from the 1940s of lower income African-American housing from the Sign Company and Gulf State Properties, Inc. in Overtown (Miami).

The E. G. Barnhill Collection contains negatives of Florida homes and birds, as well as glass plate negatives of Florida scenes. The collection also contains hand-colored postcards by Barnhill depicting Florida scenes (ca. 1910s-1930s), early Florida ephemera,  several biographical pages and scrapbooks put together by Barnhill with many resourceful historical clippings from the early half of the 20th century on pirates, Native Americans, archaeological exploits, early technology, and treasure hunting.